The University of Massachusetts Amherst

GE Biacore T200

Surface Plasmon Resonance

The real-time detection and monitoring of biomolecular binding events. Biacore can also provide quantitative information on:

  • Specificity — how specific is the binding between two molecules?
  • Affinity — how strong is the binding?
  • Kinetics — what is the rate of association/ dissociation?
  • Concentration — how much of a given molecule is present and active?
  • Multiple reactions-what are the kinetics of complex formation

Principles of Surface Plasmon Resonance (SPR) Technology





Basic SPR instrument configuration


BIAtechnology relies on the phenomenon of SPR, which occurs when surface plasmon waves are excited at a metal/liquid interface. Light is directed at, and reflected from, the side of the surface not in contact with sample, and SPR causes a reduction in the reflected light intensity at a specific combination of angle and wavelength. Biomolecular binding events cause changes in the refractive index at the surface layer, which are detected as changes in the SPR signal.

Users will need to provide their own buffers, sensor chips, etc. Please contact me to determine what is needed.