Provides transgenic, gene targeting, and other services to the research community. The core performs microinjections of DNA into fertilized embryos to generate transgenic mice. Uses cutting-edge technologies, including CRISPR/Cas9 genome editing, to generate gene knock-out or knock-in mice or other animal models. In addition, provides mouse surgery service and training, such as patient-derived xenograft (PDX), tail vein injection, and catheterization. The core is able to customize services as requested to support development of animal models of human diseases in a timely and cost-efficient manner.
Eppendorf TransferMan®4r Micromanipulators
Eppendorf CellTram®4r Air/Oil Microinjectors
Eppendorf Galaxy 48R Incubator
Thermo HERA150i Incubator
Nikon Ts2R Inverted Microscope
Nikon SMZ-800N Stereoscopes
DeNovix DS-11 Spectrophotometer
Sutter P-1000 Micropipette Puller
Narishige MF-900 Microforge
Thermo Ultra-Low TSX freezer
Syngene InGenius3 Gel Documentation
|Service Line Description||Type of Unit||Campus Users||Other Academic Institutions||Industry|
|Transgenics, Chimeras, CRISPR KO/KI Microinjection||Injection||$1,657.04||$2,179.01||$2,865.03|
|CRISPR gRNA Design||Sample||$308.33||$405.45||$533.10|
|CRISPR gRNA Synthesis||Sample||$575.75||$757.11||$955.46|
|Embryo transfer (surgical)||Transfer||$1,340.33||$1,762.53||$2,317.43|
|Embryo transfer (non-surgical)||Transfer||$804.20||$1,057.52||$1,390.46|
|DNA Extraction and PCR Genotyping||Sample||$15.48||$20.35||$26.76|
|Xenograft and Similar Surgery||Mouse||$110.52||$145.33||$191.09|
|Tail Vein Injection||Mouse||$30.00||$39.45||$51.87|
|Rates are subject to change, contact facility to verify current fees.|
- We will microinject more than 100 mouse embryos (usually around 150), culture them and perform embryo transfers to pseudopregnant females. All delivered pups will be genotyped and sequenced once they reach wean age, and then will be transferred to the principal investigator (PI). PI must obtain IACUC approval for the project before we begin the project. We cannot guarantee the expression and/or transmission of the transgene, nor that any mice will contain the desired KO or KI alleles, as this is subject to many factors (the locus, whether the mutation is deleterious, the quality of materials provided, etc.).
- We typically use hybrid embryos (B6D2F1 X B6D2F1) to perform microinjection and generate the founders. Other strains can be used (e.g., C57BL/6J or C57BL/6N) in order to create a coisogenic mouse line, however, additional costs will be applied and delays may be up to 2 months.
- The prices described in our website do not include per diem charges.
- Please acknowledge use of UMass Amherst Animal Models Core in all publications. When considering whether or not to include core laboratory members on the publications, please refer to this published guideline https://abrf.org/authorship-guidelines.