Program | Faculty
All courses carry 3 credits unless otherwise specified.
542 Immunology Laboratory
Laboratory procedures in cellular immunology, immunochemistry exploring protein chemistry of antibodies, including antibody isolation using salt precipitation, ion exchange, and molecular sieving column chromatography, spectrophotometry, SDS polyacrylamide gel electrophoresis (PAGE), comparative proteomics, western blotting, immunoprecipitation, immunodiffusion (Ouchterlony technique), enzyme-linked immunoassay (ELISA), and immunofluorescence staining. Anatomy of the lymphatic system, mouse dissection and isolation of lymphocytes from spleen and thymus; cellular immunology, including histology of mouse and human leukocytes, principles of ABO blood grouping, normal and diseased lymphoid tissue, and two-color flow cytometric analysis of lymphocyte subpopulations. Prerequisite or corequisite: course in immunology, e.g., MICROBIO 320 or ANIMLSCI 472. Mr. Webley
550 Infection and Immunity
Principles of host-parasite interactions; examination of fundamental knowledge concerning: i) infections caused by pathogenic bacteria; ii) virulence factors, such as toxins and microbial structures; iii) pathogenesis and pathology of bacterial infections; iv) nonspecific factors in host resistance and susceptibility to infectious disease; and v) immunology of microbial infection. Prerequisite: MICROBIO 320 or ANIMLSCI 472 or consent of instructor. Ms. Stuart
552 Pathogenic Bacteriology
Laboratory procedures in clinical and diagnostic bacteriology including: i) culture and physiological characteristics of the pathogenic bacteria; ii) recommended procedures for the cultivation of both the common and rare pathogens isolated from clinical material; iii) conventional and rapid biochemical methods for detection and identification of medically important bacteria; iv) serological procedures on microorganisms of prime medical concern; and v) prescribed tests for the susceptibility of bacteria to antibiotics and antimetabolites. Prerequisite: MICROBIO 312; may be taken concurrently only by consent of instructor. Ms. Stuart
560 Microbial Diversity
Exploration and interpretation of the diversity of microbial life. The role in nature of various groups of microbes; their physiology and ecology. Metabolism and energy conservation in chemoheterotrophic and chemolithotrophic bacteria and archaea. Anoxygenic and oxygenic photosynthesis. Microbial behaviors, symbiotic associations, and communities. Emphasis on anaerobes. Prerequisite: MICROBIO 310 or consent of instructor; course in biochemistry desirable. Ms. Leschine
562 Environmental Biotechnology
Traditional and molecular methods strategically applied to problems related to microbial biotechnology and environmental microbiology. Ranges from the diversity of microbial life to biodegradation. Seven general areas emphasized: 1) Statistical sampling and site characterization, 2) biomass determination, 3) enrichment techniques, 4) microbial activity measurements, 5) single cell detection in situ, 6) sequence and phylogenetic analysis followed by probe design, and 7) other modern techniques of environmental microbiology. Each are accompanied by lectures and ongoing discussions led by the respective faculty member. Ms. Leschine, Mr. Nüsslein, Mr. Petsch.
565 Laboratory in Molecular Genetics
Methodology and principles of modern molecular genetics. Microbial genetics combining classical techniques with bacteriophages and bacteria with modern PCR and recombinant DNA experiments. Introduction to genomic and structural analysis using computer methods. Emphasis on experimental design and analysis. Prerequisite: MICROBIO 330 or equivalent or consent of instructor. Mr. Burand, Mr. Sandler
Molecular biology of viruses and viral genetic systems; viral disease processes. Emphasis on polio virus, influenza, herpes viruses, the DNA tumor viruses, retroviruses (including HIV), and hepadna viruses. Prerequisite: background in cell biology, genetics, or biochemistry. Mr. Norkin
585 Concepts in Molecular Genetics
Fundamental and advanced concepts in bacterial gene regulation, with focus on bacterial responses to environmental stress. Emphasis on experimental design, and data interpretation and presentation. Prerequisites: basic coursework in biochemistry and genetics.
590B Bioinformatics Lab
An introduction to using bioinformatics to discover and test biological hypotheses. Students learn how to program in Perl, use the statistical language R, and become familiar with common algorithms and programs used in bioinformatics, particularly in the areas of genome sequence and expression analysis. Mr. Blanchard
590L Parasitology Lab
Hands-on experience in parasitological studies. Students analyze live and prepared samples of selected parasites, and the insect vectors that transmit disease. Experimental sessions involve completing parasite life cycles, inhibiting parasite growth using various drugs, and the genetic technique called RNA interference. Corequisite: MICROBIO 590S or consent of instructor. Ms. Klingbeil
Classical and modern parasitology concentrating on protozoan and worm parasites of major medical/veterinary importance. Topics include basic principles of parasitology, life cycles, epidemiology, host-parasite interactions, drug treatments and vector control programs, along with information on the basic biology, biochemistry, and genetics of selected parasites. Prerequisites: introductory course in biology; BIOCHEM 285, MICROBIO 310. Ms. Klingbeil
590W Science Writing and Reviewing
Focus on writing and reviewing scientific manuscripts and grant proposals. Students write a short research paper based on sample data and formatted for a specific journal, review a paper prepared by a faculty member, and review a grant proposal prepared by a faculty member and discuss the review in a mock study section. Students work in small groups to stimulate collaborative thinking. Credit, 2. Mr. Lopes
597B Microbial Biotechnology
Examines agricultural and industrial uses of microorganisms and genetic modification of microbes for specific purposes. Topics include history, microbial processes and products, biofuels production, single cell protein production, bioremediation, N2 fixation, Agrobacterium and plant genetics, genetically modified organisms; government regulation and social implications. Prerequisite: MICROBIO 310 or consent of instructor. Credit, 1-3. Ms. Leschine
597E Environmental Microbiology
How microorganisms interact with each other and with their environment. Microbial distribution and activities in natural systems, and their importance to ecosystem function and environmental quality. Extreme habitats; habitat-specific forces; microbial activity; microbial transformations and their impact on different environments; species diversity, detection, and control of microorganisms; and associations with higher organisms. Each basic principle followed by applied and environmental case studies. Modern techniques of environmental microbiology. Graduate students write an independent research proposal. Prerequisite: MICROBIO 310. Mr. Nüsslein.
680 Microbial Physiology
Main aspects of microbial growth, energy and biosynthesis pathways, metabolic regulation and integration of pathways into a coherent system. Emphasis on physiological diversity, global control systems governing the adaptation of microorganisms to different environmental conditions, and emerging methodologies. Prerequisite: general background in microbiology and biochemistry. Mr. Holden
690E Microbial Evolution and Ecology
Theoretical and experimental issues confronting the application of evolutionary and ecological principles to microbial populations and communities. What is currently known about microbial ecology from the level of individuals to ecosystems as well as issues still unresolved. Mr. Schloss
696 Independent Study
Research under direction of a faculty member. Credit, 1-8.
697D Special Topics: Acid Mine Drainage
Critical reviews and discussions of the current literature dealing with biogeochemistry of acid mine drainage (AMD). Establishes a broad understanding of the current literature and the basics of the biogeochemistry of AMD, and the biogeochemical processes related to AMD production and attenuation. AMD studied as a model for the interaction of microbial, geological, and hydrochemical systems. Credit, 1. Mr. Nüsslein
Introduction to the biological, computational, and statistical foundations necessary for bioinformatics-related research. Includes biological databases, DNA and protein sequence analysis, structure-based analysis, expression analysis, and genetic mapping. Cross-listed in Mathematics and Statistics, Microbiology and Computer Science. Mr. Blanchard
699 Master’s Thesis
791 Departmental Seminar
Reports and discussion of pertinent literature and research. Required of all microbiology graduate majors each semester in residence. Credit, 1.
796 Independent Study
Research under direction of a faculty member. Credit, 1-7.
797B Microbial Aspects of Biogeochemistry
Studies the active subsurface biosphere in sedimentary basins, to advance understanding of: 1) the forms of metabolic processes employed by such populations, and limits on the activity of subsurface microbial communities; 2) the origin, survival, and dormancy of slowly-growing subsurface microorganisms far removed from earth surface influences; 3) the geochemical, mineralogical, and molecular signatures that subsurface organisms may imprint on rocks and sediments; and 4) the impact that active modern populations may have on overprinting of paleoenvironmental signatures preserved in ancient sediments and rocks. Credit, 1. Mr. Nüsslein
797E Microbial Ecology Journal Club
Presentations and discussions of important research papers from the current literature. Emphasis on either new strategies to address fundamental issues in microbial ecology or on hypothesis-based microbial ecology research. All participants should expect to lead a discussion. Presentations start with postdoctoral and senior graduate students. Credit, 1. Mr. Nüsslein
797J Immunology Journal Club
Critical review of the scientific literature of benefit to students and faculty. Students make one presentation, choosing their own article from selected journals, with the guidance of an immunology faculty member if desired. The major topics include, but are not limited to, programmed cell death, innate immune response, cellular and molecular immunology, virus immunology, and receptor-mediated signaling. Credit, 1. Mr. Anguita, Ms. Baldwin, Mr. Black, Mr. Goldsby, Ms. Osborne, Ms. Telfer, Mr. Webley
797P Molecular Mechanisms in Pathogenesis
Explores through current primary literature some of the complex processes used by microbial pathogens to establish themselves in a host and to gain nutrients, subsequently causing host cell damage and disease, and to evade the host’s defense. Includes discussion of the modern molecular biology, genetics, and biochemistry tools to study these processes. Ms. Klingbeil
899 Doctoral Dissertation