Robert E. Levin
Professor
Food safety, detection of mutagens and carcinogens; microbial food spoilage enzymes; immuno assays, fermentation technology and PCR detection and quantitation of E. coli O157:H7, Listeria monocytogenes, vibrio parahaemolyticus and V. vulnificus in seafood.
Office: Room 346, Chenoweth Laboratory
Off. Tel.: (413) 545-0187
Email: relevin@foodsci.umass.edu
Curriculum Vitae |
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Publications
Abolmaaty, A, C. Vu, J. Oliver, and R. E. Levin. 2000. Development of a new lysis solution for releasing genomic DNA from bacterial cells for DNA amplification by polymerase chain reaction. Microbios. 101: 181-189.
Ferenc, J., Oliver, J., Witkowski, R., McLandsborough, L. and Levin, R. E. 2000. Studies on the growth of Escherichia coli O157:H7 strains at 45oC. J. Food Protection. 63: 1173-1178.
Guan, J. and Levin, R. E. 2002. Quantitative Detection of Escherichia coli O157:H7 in ground beef by the polymerase chain reaction. Food Microbiol. 19: 159-165.
Abolmaaty, A. and Levin, R. E. 2002. Development of a microslide aglutination assay with the aid if an inexpensive projection microscope. J. Microbiol. Methods. 51: 421-423.
Zhang, Y.,Lu, H. and Levin, R. E. 2003. Enhanced storage-life of fresh haddock fillets with stabilized sodium chlorite in ice. Food Microbiol. 20: 87-90.
Guan, J. and Levin, R. E. 2002. Quantitative detection of Escherichia coli O157:H7 in ground beef by immunomagnetic separation and polymerase chain reaction. Food Biotechnol. 16: (2) 135-144.
Guan, J. and Levin, R. E. 2002. Sensitive and rapid detection of Escerichia coli O157:H7 in ground beef by nested PCR incorporating immunomagnetic separation. Food Biotechnol. 16: (2) 145-154.
Guan, J. and Levin, R. E. 2002. Quantitative detection of Escherichia coli O157:H7 in ground beef by immunomagnetic separation and competitive polymerase chain reaction. Food Biotechnol. 16: (3) 155-166.
Levin, R. E. 2003. Application of the polymerase chain reaction for detection of Listeria monocytogenes in foods: a review of methodology. Food Biotechnology. 17: (2) 99-116.
Kim, J. and Levin R. E. 2003. Influence of caffeine on the repair of mitomycin C induced DNA damage. Food Biotechnology. 17: (2) 151-161.
Levin, R. E. 2003. Application of random amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PGFE) analysis to Listeria monocytogenes: A review of methodology and results. Food Biotechnol. 17: (3) 203.
Levin, R. E. 2004. The application of real-time PCR to food and agricultural systems. A review. Food Biotechnol. 18(1):97-133.
Wang, S. and Levin, R. E. 2004. Quantitative determination of Vibrio parahaemolyticus by polymerase chain reaction. Food Biotechnol. 18: (3) 279-287.
Wang, S. and Levin, R. E. 2005. Quantification of Vibrio vulnificus using the polymerae chain reaction. Food Biotechnol. 19(1):27-35.
Levin, R. E. 2005. Vibrio vulnificus, a notably lethal human pathogen derived from seafood: a review of its pathogenicity, subspecies characterization, and molecular methods of detection. Food Biotechnol. 19(1):69-93.
Wang, S. and Levin, R. E. 2005. Quantification of Vibrio vulnificus using the polymerae chain reaction. Food Biotechnol. 19(1):27-35.
Discrimination of viable vibrio vulnificus cells from dead cells in real-time PCR. 2005. J. Microbiol. Methods. On-line.
Cao, J. k, Witkowski, R. k, Lu, H., Abolmaaty, A., Lu, S., and Levin, R. E. 2005. Detection, enumeration, and RAPD analysis of Listeria monocytogenes isolates in fish derived from retail sources in Western Massachusetts. Food Biotechnol. 19: 145-160.
Vattem, D. A., Jang, H-D, Levin, R. and Shetty, K. Synergism of cranberry phenolics with ellagic acid and rosmarinic acid for antigenic and DNA-protection functions. Submitted to J. Fd. Biochem. In press.
Cao, J., Cronin, C.,Mclandsborough,L. and .Levin, R. E. 2005. Effects of primers and Taq polymerase on randomly amplified polymorphic DNA analysis for typing Listeria monocytogenes from the environment of a shrimp processing plant. Food Biotechnol. 19(3):217-226.
Wang, S. and Levin, R. E. 2005. Quantitative detection of Vibrio vulnificus by competitive polymerase chain reaction. Food Biotechnol. 19(3):193-204.
Cao, J., Clark, M., Witkowski, R., Lu, H., Sayedahman, A., Levin, R. E. ,McLandsborough, L. A. 2006. Concentration and tracking of Listeria monocytogenes strains in a seafood-processing environment using a most-probable number enrichment procedure and randomly amplified polymorphic DNA analysis. J. Food Protection 69(3):489-494.
Lu, S., Liu, B., Cao, J., Zhou,B., and R. E. Levin. 2006. Incidence and enumeration of
Vibrio parahaemolyticus in shellfish from two retail sources and the genetic diversity of isolates as determined by RAPD-PCR analysis. Food Biotechnol. 20:193-210.
