Lynmarie K. Thompson

Associate Professor of Chemistry, University of Massachusetts
Director, Chemistry Biology Interface Training Program

L. Thompson Chemistry Dept Website
Chemistry Biology Interface Training Program

Ph.D.: Yale University
Postdoctoral Training: Massachusetts Institute of Technology
Honors: National Science Foundation Young Investigator, Cottrell Scholar

Structure and Mechanism of Membrane Proteins

Membrane proteins are key players in the essential cellular processes of energy and signal transduction. Our laboratory is interested in understanding the molecular mechanisms of such processes: for instance, how is a signal transmitted across a membrane?

The transmembrane receptors of bacterial chemotaxis bind specific attractant molecules and transmit this information across the membrane to direct the swimming of the bacterium. Ligand binding to this receptor is thought to cause a conformational change that propagates the signal across the membrane. However, as with most membrane proteins, the traditional tools of structural biology have not been able to provide structures of the intact receptor to follow this conformational change. We are using recently developed solid-state NMR methods to measure selected distances in membrane-bound proteins, providing structural information that can map a ligand binding site or test a proposed mechanism.

We have used a powerful site-directed distance measurement strategy to measure helix-helix distances in the periplasmic domain of the intact serine receptor which are consistent in magnitude with a proposed ligand-induced piston mechanism. Additional distance measurements are in progress to map the type of structural change: does ligand-binding induce translation, rotation, or pivoting motions of the helices? By measuring the effect of ligand-binding on helix-helix distances throughout the receptor, these studies are providing a molecular picture of how a protein transmits a signal across a membrane.

Our overall strategy is to combine site-directed NMR distance measurements with other biophysical approaches to probe the structure and mechanism of membrane proteins. Membrane proteins are both tremendously important (as pharmaceutical targets for example) and poorly understood, making this an area rich in opportunities for exciting research.

Representative publications:

Gallagher, G. J., Hong, M., and Thompson, L. K. (2004) " Solid-state NMR spin diffusion for measurement of membrane-bound peptide structure: Gramicidin A ", Biochemistry 43 , 7899-7906. [PubMed]

Thompson, L. K. (2003) " Unraveling the secrets of Alzheimer's ß-amyloid fibrils ", invited commentary for Proc. Nat. Acad. Sci USA 100 , 383-385. [PubMed]

Thompson, L. K. (2002) " Solid-state NMR studies of the structure and mechanisms of proteins ", Current Opinion in Structural Biology 12 , 661-669. [PubMed]

Isaac, B., G. J. Gallagher, Y. S. Balazs and L. K. Thompson (2002). "Site-directed rotational resonance solid-state NMR distance measurements probe structure and mechanism in the transmembrane domain of the serine bacterial chemoreceptor." Biochemistry 41(9): 3025-36.

Murphy, O. J., 3rd, X. Yi, R. M. Weis and L. K. Thompson (2001). "Hydrogen exchange reveals a stable and expandable core within the aspartate receptor cytoplasmic domain." J Biol Chem 276(46): 43262-9.

Murphy, O. J., III, Kovacs, F. A., Sicard, E., & Thompson, L. K. (2001) "Site-directed solid-state NMR measurement of a ligand-induced conformational change in the serine bacterial chemoreceptor", Biochemistry 40, 1358-1366.

Balazs, Y. S. & Thompson, L. K. (1999) "Practical methods for solid-state NMR distance measurements on large biomolecules: constant-time rotational resonance", J. Mag. Res. 139, 371-376.

Kumashiro, K.K., Schmidt-Rohr, K., Murphy, O.J., III, Ouellette, K.L., Cramer, W.A. and Thompson, L.K. (1998) A Novel Tool for Probing Membrane Protein Structure: Solid-State NMR with Proton Spin Diffusion and X-Nucleus Detection. J. Am. Chem. Soc. 120, 5043-5051.

Wang, J., Balazs, Y.S. and Thompson, L.K. (1997) Solid-state REDOR NMR distance measurements at the ligand site of a bacterial chemotaxis membrane receptor. Biochemistry 36, 1699-1703.

Seeley, S.K., Wittrock, G.K., Thompson, L.K. and Weis, R.M. (1996) Oligomers of the Cytoplasmic Fragment from the Escherichia coli Aspartate Receptor Dissociate Through an Unfolded Transition State. Biochemistry 35, 16336-16345.

Seeley, S.K., Weis, R.M. and Thompson, L.K. (1996) The Cytoplasmic Fragment of the Aspartate Receptor Displays Globally Dynamic Behavior. Biochemistry 35, 5199-5206.