Sallie W. Smith Schneider

Scientist, Pioneer Valley Life Sciences Institute
Adjunct Research Assistant Professor of Veterinary and Animal Sciences, University of Massachusetts

Email: ssmith@vasci.umass.edu
S. Schneider's PVLSI Web Site
S.Schneider Veterinary & Animal Sciences Web Site

Ph.D.: University of Massachusetts, Amherst
Postdoctoral Training: Harvard Medical School

Signaling During Mammary Gland Development and Carcinogenesis

Development of the mature mammary gland requires numerous signaling pathways engaged by secreted factors and cell:cell interactions. My overall interest is in studying the signaling pathways that control normal and abnormal development in the mammary gland, as well as the role of communication between various cellular compartments in these processes. In the past few years we have focused on the role of Notch receptor proteins during normal and abnormal development. The Notch family of proteins is comprised of four extracellular membrane-associated receptors (Notch 1-4) whose structure and function have been highly conserved throughout evolution. Signaling through these receptors is initiated by ligand on adjacent cells, which results in activation through proteolytic cleavage events at the membrane. The cytoplasmic domain is released and translocates to the nucleus where it participates in numerous protein:protein interactions which affect transcription. Transgenic studies have indicated that deregulated expression of the Notch4 receptor results in a retarded growth of the epithelial cell compartment of the mammary gland and a block in lobulo-alveolar development. Furthermore, these mice develop breast tumors within three to six months.

My lab has been studying signaling pathways induced by Notch receptors and how these pathways interact with a number of other signaling pathways involved in mammary gland development. I am particularly interested in the interplay between Notch signaling and steroid receptor transactivation, p53 activation, and activation of growth factor signaling (i.e. EGF and TGF?). As a member of the Baystate/UMASS Research Institute, I will be presented with a unique and exciting opportunity to move my research in a more translational direction.

Representative publications:

Leon O. Murphy, Sallie Smith, Rey-Huei Chen, Diane C. Fingar, and John Blenis (2002) Regulation of the immediate early gene product c-Fos by ERK and RSK: a mechanism for sensing and propagating ERK signal duration. Nature Cell Biology 4(8):556-564.

Sallie W. Smith and Anneke C. Blackburn (2002) Critical Commentary: Women's Oncology Review 2(1):37-40

Kulmeet K. Sandhu, Joseph M. Simard, Catherine M. McIntosh, Sallie W. Smith, and Vincent M. Rotello. (2002) Gold nanoparticle-mediated transfection of mammalian cells. Bioconjugate Chemistry 13(1):3-6

Carla Ana Gordo, Chang Li He, Sallie Smith, Rafael A. Fissore (2001) Mitogen activated protein kinase plays a significant role in metaphase II arrest, spindle
morphology, and maintenance of maturation promoting factor activity in bovine oocytes. Mol. Repro.and Dev.59:106-114

Grant Morgan, Sallie W. Smith, John Pak, Ann Marshak-Rothstein, Rafael Fissore, Barbara A. Osborne (1999) Characterization of a mutant T cell hybridoma line with defects in TCR-mediated apoptotic pathway. Cell Death and Diff. 6:36-47.

Sallie W. Smith and Barbara A.Osborne (1997) Private pathways to a common death. J. NIH Research 9:33-37.

Sallie W. Smith and Barbara A. Osborne (1995) Molecular events in thymocyte apoptosis. In: Current Topics in Micro. & Immunol., Vol.200:147-162.

Zheng-Gang Liu*, Sallie W. Smith*, Kelly A. McLaughlin, Lawrence M. Schwartz, Barbara A. Osborne (1994) "Apoptotic signals delivered through the T-cell receptor of a T-cell hybrid require the immediate-early gene nur77" Nature 367, 281-284. (*Contributed equally to this work)

Scott W. Lowe, Earlene M. Schmitt, Sallie W. Smith, Barbara A. Osborne, Tyler Jacks (1993) "p53 is required for radiation-induced apoptosis in mouse thymocytes." Nature 362, 847-849.

Lawrence M. Schwartz, Sallie W. Smith, Margaret E.E. Jones, Barbara A. Osborne (1993) "Do all programmed cell deaths occur via apoptosis?" Proc. Natl. Acad. Sci. 90, 980-984.