Steven J. Sandler

Associate Professor of Microbiology, University of Massachusetts
Interim Department Head of Microbiology

S. Sandler Microbiology Dept Website

Ph.D.: University of California at Berkeley
Postdoctoral Training: Advanced Genetic Sciences Inc.; University of California at Berkeley

The Molecular Mechanisms of DNA Replication and Homologous Recombination

My research interests focus on the broad area of DNA transactions. This includes how DNA is repaired, recombined, and replicated at the molecular level. One group of projects uses genetics to address why and how the process of DNA replication is needed to do recombination. This is particularly new and exciting because most mainstay models of recombination require little or no DNA replication. The other projects ask how organisms that grow in extreme environments recombine and replicate their DNA. Here we study Sulfolobus solfataricus. It grows in the laboratory at 75°C and pH 3 and is amenable to most current molecular genetic techniques. We are also interested in surveys of Archaeans from different environments: soil, ocean, swamps, etc., to study the biodiversity of the RecA family of proteins at the molecular level and for use as a phylogenetic marker.

Representative publications:

Ruethairat Boonsombat, Su-Ping Yeh, Amy Milne and Steven J. Sandler (2006) A novel dnaC mutation that suppresses priB rep mutant phenotypes in Escherichia coli K-12. Molecular Microbiology 60(4) : 973-983.

Renzette, Nicholas, Nathan Gumlaw, Jared T. Nordman, Marlee Krieger, Su-Ping Yeh, Edward Long, Richard Centore, Ruethairat Boonsombat and Steven J. Sandler (2005) Localization of RecA in E. coli K-12 Using RecA-GFP. Molecular Microbiology 57(4): 1074-1085.

Sandler, Steven J. (2005) Requirements for Replication Restart Proteins during Constitutive Stable DNA Replication (cSDR) in E. coli K-12. Genetics 169 (4) : 1799-1806.

Hinds, Tania and Steven J. Sandler (2004) Allele specific synthetic lethality between priC and dnaA ts alleles at the permissive temperature of 30C in E. coli K-12. BMC Microbiology 4: 47-58.

McCool, Jesse D., Edward Long, Joseph F. Petrosino, Hilary A. Sandler, Susan M. Rosenberg and Steven J. Sandler (2004) Measurement of SOS expression in individual Escherichia coli K-12 cells using fluorescence microscopy. Molecular Microbiology 53:1343-1357.

McCool, Jesse D., Christopher C. Ford and Steven J. Sandler (2004) A dnaT mutant with similar phenotype to a priA2::kan mutant in Escherichia coli K-12. Genetics 167:569-578.

Lloyd, Jonathan R., C. Leang, A. L. Hodges Myerson, M. V. Coppi, S. Cuifo, B. Methe, S. J. Sandler, D. R. Lovley (2002) Biochemical and genetic characterization of CycP, a periplasmic C-type cytochrome in Geobacter sulfurreducens. Biochemical Journal 369: 153-61.

McCool, Jesse D. and Steven J. Sandler (2001) Effects of mutations involving cell division, recombination, and chromosome dimer resolution on a priA2::kan mutant. Proceedings of the National Academy of Science, USA 98(15): 8203-8210.

Sandler, S. J., J. D. McCool, T. T. Do and R. U. Johansen (2001). "PriA mutations that affect PriA-PriC function during replication restart." Mol Microbiol 41(3): 697-704.

Coppi, M. V., C. Leang, S. J. Sandler and D. R. Lovley (2001). "Development of a genetic system for Geobacter sulfurreducens." Appl Environ Microbiol 67(7): 3180-7.

McCool, J. D. and S. J. Sandler (2001). "Effects of mutations involving cell division,
recombination, and chromosome dimer resolution on a priA2::kan mutant." Proc Natl Acad Sci U S A 98(15): 8203-10.

Cox, M. M., M. F. Goodman, K. N. Kreuzer, D. J. Sherratt, S. J. Sandler and K. J. Marians (2000). "The importance of repairing stalled replication forks." Nature 404(6773): 37-41.

Sandler, S. J. and K. J. Marians (2000). "Role of PriA in replication fork reactivation in Escherichia coli." J Bacteriol 182(1): 9-13.

Sandler, S. J. (2000). "Multiple genetic pathways for restarting DNA replication forks in Escherichia coli K-12." Genetics 155(2): 487-97.

Seitz, E. M., J. P. Brockman, S. J. Sandler, A. J. Clark and S. C. Kowalczykowski (1998). "RadA protein is an archaeal RecA protein homolog that catalyzes DNA strand exchange." Genes Dev 12(9): 1248-53.

Sandler, S. J., P. Hugenholtz, C. Schleper, E. F. DeLong, N. R. Pace and A. J. Clark (1999). "Diversity of radA genes from cultured and uncultured archaea: comparative analysis of putative RadA proteins and their use as a phylogenetic marker." J Bacteriol 181(3): 907-15.

Sandler, S. J., K. J. Marians, K. H. Zavitz, J. Coutu, M. A. Parent and A. J. Clark (1999). "dnaC mutations suppress defects in DNA replication- and recombination-associated functions in priB and priC double mutants in Escherichia coli K-12." Mol Microbiol 34(1): 91-101.

Liu, J., L. Xu, S. J. Sandler and K. J. Marians (1999). "Replication fork assembly at recombination intermediates is required for bacterial growth." Proc Natl Acad Sci U S A 96(7): 3552-5.