The University of Massachusetts Amherst

Stephen Eyles

Extension Associate Professor

Our goal in the Mass Spectrometry Core Facility is to engage researchers and provide state of the art analytical instrumentation, analytical services, and expertise in mass spectrometry for UMass Amherst, neighboring scientific communities, and their collaborators. The Core currently serves the needs of more than 50 research groups across campus with active life science research interests, ranging from confirmation of organic synthesis products and synthetic polymer studies to analysis of modifications in proteins and macromolecular complexes, as well as protein folding, assembly, and dynamics investigated through chemical labeling and cross-linking. Staff can provide instrument training to students and researchers, as well as expert advice on experimental design and data interpretation. Life science-focused research in the Center includes projects to identify and quantify proteins in complex matrices as well as metabolites. The goal is to provide expertise, training, and instrumental assistance to researchers so that they can optimize usage of the equipment and maximize the utility of data obtained.

Current Research

Protein dynamics is critical to understanding the processes involved in protein homeostasis, for example, the mechanisms of chaperone action to maintain healthy folded proteins or the mechanisms of protein oligomerization leading to aggregation or formation of amyloid plaques. Current research interests include applying mass spectrometry methodologies to investigate protein-ligand and protein-protein interactions as well as protein dynamics, through the use of hydrogen-deuterium exchange and chemical cross-linking. The multitude of uses for mass spectrometry in the biophysical arena is rapidly evolving, and IALS at UMass Amherst is at the forefront of this technology, developing new techniques to study the fate of proteins and other biomolecules in and out of the cell. The Mass Spectrometry Core Facility makes leading-edge technology available to researchers in order to address these critically important questions. See the MS Core website for further details about instrumentation available.

Learn more at https://www.umass.edu/ials/mass-spectrometry

Academic Background

  • BA/MA University of Oxford, UK, 1990
  • DPhil University of Oxford, UK, 1995
  • Postdoctoral Training: University of Massachusetts Amherst
Limpikirati PK, Zhao B, Pan X, Eyles SJ, Vachet RW. Covalent Labeling/Mass Spectrometry of Monoclonal Antibodies with Diethylpyrocarbonate: Reaction Kinetics for Ensuring Protein Structural Integrity. J Am Soc Mass Spectrom. 2020 Jun 3;31(6):1223-1232. doi: 10.1021/jasms.0c00067. Epub 2020 Apr 30. PubMed PMID: 32310649; PubMed Central PMCID: PMC7370534
Deol KK, Eyles SJ, Strieter ER. Quantitative Middle-Down MS Analysis of Parkin-Mediated Ubiquitin Chain Assembly. J Am Soc Mass Spectrom.2020 May 6;31(5):1132-1139. doi: 10.1021/jasms.0c00058. Epub 2020 Apr 28. PubMed PMID: 32297515; PubMed Central PMCID: PMC7333183.
Fernandez K, Spielbauer KK, Rusheen A, Wang L, Baker TG, Eyles S, Cunningham LL. Lovastatin protects against cisplatin-induced hearing loss in mice. Hear Res. 2020 Apr;389:107905. doi: 10.1016/j.heares.2020.107905. Epub 2020 Feb 6. PubMed PMID: 32062294; PubMed Central PMCID: PMC7080598.
Duan Q, Liu MJ, Kita D, Jordan SS, Yeh FJ, Yvon R, Carpenter H, Federico AN, Garcia-Valencia LE, Eyles SJ, Wang CS, Wu HM, Cheung AY. FERONIA controls pectin- and nitric oxide-mediated male-female interaction. Nature. 2020 Mar;579(7800):561-566. doi: 10.1038/s41586-020-2106-2. Epub 2020 Mar 18. PubMed PMID: 32214247.
Bednarski DM, Lantz EE, Bobst CE, Eisenhut AR, Eyles SJ, Fey JP. Sterilization of epidermal growth factor with supercritical carbon dioxide and peracetic acid; analysis of changes at the amino acid and protein level. Biochim Biophys Acta Proteins Proteom. 2020 Feb;1868(2):140334. doi: 10.1016/j.bbapap.2019.140334. Epub 2019 Nov 29. PubMed PMID: 31786473.
Li X, Eyles SJ, Thompson LK. Hydrogen exchange of chemoreceptors in functional complexes suggests protein stabilization mediates long-range allosteric coupling. J Biol Chem. 2019 Nov 1;294(44):16062-16079. doi: 10.1074/jbc.RA119.009865. Epub 2019 Sep 10. PubMed PMID: 31506298; PubMed Central PMCID: PMC6827319.
Liang C, Savinov SN, Fejzo J, Eyles SJ, Chen J. Modulation of Amyloid-β42 Conformation by Small Molecules Through Nonspecific Binding. J Chem Theory Comput. 2019 Oct 8;15(10):5169-5174. doi: 10.1021/acs.jctc.9b00599. Epub 2019 Sep 4. PubMed PMID: 31476124; PubMed Central PMCID: PMC6783347.
Martin CB, Chaplin VD, Eyles SJ, Knapp MJ. Protein Flexibility of the α-Ketoglutarate-Dependent Oxygenase Factor-Inhibiting HIF-1: Implications for Substrate Binding, Catalysis, and Regulation. Biochemistry. 2019 Oct 1;58(39):4047-4057. doi: 10.1021/acs.biochem.9b00619. Epub 2019 Sep 20. PubMed PMID: 31499004; PubMed Central PMCID: PMC7003530.
 
Contact Info

Director, Mass Spectrometry Center
Department of Biochemistry and Molecular Biology
Life Science Laboratories S503
240 Thatcher Way
Amherst, MA 01003-9292

(413) 577-1528
eyles@umass.edu

massspec.biochem.umass.edu/