The University of Massachusetts Amherst

Lisa Minter

Assistant Professor

My area of research focuses on the role NOTCH1 signaling plays in autoimmune responses. We are currently investigating how NOTCH signaling contributes to pathology and disease progression during immune-mediated bone marrow failure (BMF). We have established two new models of BMF, both of which are highly representative of the human immune-meditated BMF syndrome, Aplastic Anemia. In our first model, we transfer bulk splenocytes from a parental C57BL/6 strain into recipient mice that are the F1 hybrid progeny of a C57BL/6 x BALB/c cross. The result is a robust graft-versus-host response whereby the transferred splenocytes selectively target the bone marrow for destruction. The onset of disease is precipitous and symptoms include loss of repopulating stem cells from the bone marrow and immune cells from the periphery, infiltration of destructive CD4+ and CD8+ T cells into the recipient BM, as well as increased levels of the pro-inflammatory cytokines, interferonγ and TNF in the circulation. Our “humanized” model utilizes a recently developed transgenic mouse strain, Nod/SCID/IL2Rγcnull (NSG), in which BMF is induced following the transfer of human peripheral blood mononuclear cells.

Current Research
Using our two established models of BMF, we are continuing to probe the role of NOTCH1signaling in Aplastic Anemia. We are currently investigating how NOTCH1 cooperates with proteins essential for T cell activation to drive disease pathogenesis, as well as how novel target genes of NOTCH1 may contribute to Aplastic Anemia in the context of aberrant NOTCH1 signaling. We are also pursuing interdisciplinary collaborations we hope will allow us to specifically target NOTCH1signaling in the immune system using novel nanotechnology-based delivery platforms.

Learn more at www.vasci.umass.edu/research-faculty/lisa-m-minter

Academic Background

  • PhD University of Massachusetts, 2001
  • Postdoctoral training: UMass Amherst
Chandiran K, Lawlor R, Pannuti A, Perez GG, Srinivasan J, Golde TE, Miele L, Osborne BA, Minter LM. Notch1 primes CD4 T cells for T helper type I differentiation through its early effects on miR-29. Molecular immunology. 2018; 99:191-198. NIHMSID: NIHMS974497 PubMed [journal] PMID: 29807327, PMCID: PMC6281167
Ozay EI, Sherman HL, Mello V, Trombley G, Lerman A, Tew GN, Yadava N, Minter LM. Rotenone Treatment Reveals a Role for Electron Transport Complex I in the Subcellular Localization of Key Transcriptional Regulators During T Helper Cell Differentiation. Frontiers in immunology. 2018; 9:1284. PubMed [journal] PMID: 29930555, PMCID: PMC5999735
Hossain F, Majumder S, Ucar DA, Rodriguez PC, Golde TE, Minter LM, Osborne BA, Miele L. Notch Signaling in Myeloid Cells as a Regulator of Tumor Immune Responses. Frontiers in immunology. 2018; 9:1288. PubMed [journal] PMID: 29915603, PMCID: PMC5994797
Sanidad KZ, Yang H, Wang W, Ozay EI, Yang J, Gu M, Karner E, Zhang J, Kim D, Minter LM, Xiao H, Zhang G. Effects of Consumer Antimicrobials Benzalkonium Chloride, Benzethonium Chloride, and Chloroxylenol on Colonic Inflammation and Colitis-Associated Colon Tumorigenesis in Mice. Toxicological sciences : an official journal of the Society of Toxicology. 2018; 163(2):490-499. PubMed [journal] PMID: 29514330
Yang H, Wang W, Romano KA, Gu M, Sanidad KZ, Kim D, Yang J, Schmidt B, Panigrahy D, Pei R, Martin DA, Ozay EI, Wang Y, Song M, Bolling BW, Xiao H, Minter LM, Yang GY, Liu Z, Rey FE, Zhang G. A common antimicrobial additive increases colonic inflammation and colitis-associated colon tumorigenesis in mice. Science translational medicine. 2018; 10(443). NIHMSID: NIHMS998034 PubMed [journal] PMID: 29848663, PMCID: PMC6343133
Ran Y, Hossain F, Pannuti A, Lessard CB, Ladd GZ, Jung JI, Minter LM, Osborne BA, Miele L, Golde TE. γ-Secretase inhibitors in cancer clinical trials are pharmacologically and functionally distinct. EMBO molecular medicine. 2017; 9(7):950-966. PubMed [journal] PMID: 28539479, PMCID: PMC5494507
Sgolastra F, Backlund CM, Ilker Ozay E, deRonde BM, Minter LM, Tew GN. Sequence segregation improves non-covalent protein delivery. Journal of controlled release: official journal of the Controlled Release Society. 2017; 254:131-136. NIHMSID: NIHMS891701 PubMed [journal] PMID: 28363520, PMCID: PMC5568762
Sarapas JM, Backlund CM, deRonde BM, Minter LM, Tew GN. ROMP- and RAFT-Based Guanidinium-Containing Polymers as Scaffolds for Protein Mimic Synthesis. Chemistry (Weinheim an der Bergstrasse, Germany). 2017; 23(28):6858-6863. NIHMSID: NIHMS889375 PubMed [journal] PMID: 28370636, PMCID: PMC5551038
 
Contact Info

Department of Veterinary and Animal Sciences
427K Integrated Science Building
661 North Pleasant Street
Amherst, MA 01003

(413) 545-6327
lminter@vasci.umass.edu

www.vasci.umass.edu/research-faculty/lisa-m-minter