Igor A. Kaltashov
Bioanalytical chemistry; biological and medicinal applications of mass spectrometry; structure, properties, delivery and mechanism of action of protein-based therapeutics.
Biopharmaceuticals are a unique class of medicines due to their extreme structural complexity. The structure of these therapeutic proteins is critically important for their efficacy and safety, and the ability to characterize it at various levels (from sequence to conformation) is critical not only at the quality control stage, but also throughout the discovery and design stages. Biological mass spectrometry (MS) offers a variety of approaches to study structure and behavior of complex protein drugs and has already become a default tool for characterizing the covalent structure of protein therapeutics, including sequence and post-translational modifications. Recently, MS-based methods have also begun enjoying a dramatic growth in popularity as a means to provide information on higher order structure and dynamics of biotechnology products. In particular, several MS-based methods recently developed in our laboratory offer a convenient way to assess the integrity of protein conformation and monitor interactions of protein drugs with their therapeutic targets and other physiological partners using simple model systems. MS-based methods are also applied to study pharmacokinetics of biopharmaceutical products, where they begin to rival traditional immunoassays. MS already provides valuable support to all stages of development of biopharmaceuticals, from discovery to post-approval monitoring, and its impact on the field of biopharmaceutical analysis will undoubtedly continue to grow.
Learn more at www.chem.umass.edu/people/kaltashovlab/
- Postdoctoral Training: Johns Hopkins University School of Medicine
- PhD University of Maryland Baltimore County
- MS Moscow Institute of Physics and Technology